期刊
APPLIED SCIENCES-BASEL
卷 9, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/app9224784
关键词
pancreatic cancer; resection; circulating microRNAs; biomarkers; progression free survival; immune cells
类别
资金
- Lombardi Comprehensive Cancer Center
- NIH [P30 CA51008]
- Ruesch Center for the Cure of GI Cancers
- Living with Hope Foundation
- International Fulbright Science and Technology award
- Croatian Graduate Student Foundation Award
Featured Application Circulating prognostic biomarker in pancreatic cancer. Abstract Clinical follow-up aided by changes in the expression of circulating microRNAs (miRs) may improve prognostication of pancreatic ductal adenocarcinoma (PDAC) patients. Changes in 179 circulating miRs due to cancer progression in the transgenic Kras(G12D/+); Trp53(R172H/+); P48-Cre (KPC) animal model of PDAC were analyzed for serum miRs that are altered in metastatic disease. In addition, expression levels of 250 miRs were profiled before and after pancreaticoduodenectomy in the serum of two patients with resectable PDAC with different progression free survival (PFS) and analyzed for changes indicative of PDAC recurrence after resection. Three miRs that were upregulated >= 3-fold in progressive PDAC in both mice and patients were selected for validation in 26 additional PDAC patients before and after resection. We found that high serum miR-125b-5p and miR-99a-5p levels after resection are significantly associated with shorter PFS (HR 1.34 and HR 1.73 respectively). In situ hybridization for miR detection in the paired resected human PDAC tissues showed that miR-125b-5p and miR-99a-5p are highly expressed in inflammatory cells in the tumor stroma, located in clusters of CD79A expressing cells of the B-lymphocyte lineage. In conclusion, we found that circulating miR-125b-5p and miR-99a-5p are potential immune-cell related prognostic biomarkers in PDAC patients after surgery.
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