期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 5, 期 11, 页码 6082-6088出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.9b01413
关键词
silk fibroin; CpG oligodeoxynucleotides; drug delivery; immunostimulation
资金
- National Natural Science Foundation of China [21907041, 21574059, 31700708]
- Natural Science Foundation of Jiangsu Province [BK 20170202, BK 20170181]
- national first-class discipline program of Light Industry Technology and Engineering [LITE2018-20]
CpG oligodeoxynucleotides (ODNs) have attracted increasing attention as immunotherapeutic agents. However, efficient transfection of CpG ODNs into the immune cells remains a big challenge. In this study, for the first time, we reported that silk fibroin (SF) could function as an efficient carrier for CpG ODNs. A novel strategy was developed to prepare SF-CpG ODNs nanoparticles (NPs) based on self-assembly of SF. The as-prepared SF-CpG NPs were spherical in shape and were uniformly dispersed. SF-CpG NPs exhibited good stability and biocompatibility. SF-CpG NPs possessed significantly enhanced (7 folds) cellular uptake compared with CpG ODNs. Release of CpG ODNs from SF-CpG NPs was accelerated in environment-mimicking TLR9-localized endo/lysosome. SF-CpG NPs stimulated about four folds higher levels of immune cytokines and nitric oxide compared with CpG ODNs. Our results suggested that SF notably improved the CpG ODNs delivery. SF-CpG NPs have strong potential in immunotherapy.
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