期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 152, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/60034
关键词
Biochemistry; Issue 152; Traction force microscopy; monolayer stress microscopy; mechanobiology; gap junction; Cx43; intercellular stress
资金
- University of Central Florida start-up funds
- National Heart, Lung, And Blood Institute of the National Institute of Health [K25HL132098]
Endothelial cells have been established to generate intercellular stresses and tractions, but the role gap junctions play in endothelial intercellular stress and traction generation is currently unknown. Therefore, we present here a mechanics-based protocol to probe the influence of gap junction connexin 43 (Cx43) has on endothelial biomechanics by exposing confluent endothelial monolayers to a known Cx43 inhibitor 2,5-dihydroxychalcone (chalcone) and measuring the impact this inhibitor has on tractions and intercellular stresses. We present representative results, which show a decrease in both tractions and intercellular stresses under a high chalcone dosage (2 mu g/mL) when compared to control. This protocol can be applied to not just Cx43, but also other gap junctions as well, assuming the appropriate inhibitor is used. We believe this protocol will be useful in the fields of cardiovascular and mechanobiology research.
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