期刊
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
卷 21, 期 -, 页码 68-75出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jgar.2019.10.019
关键词
Phage therapy; biofilm degradation; Herelleviridae family; SPO1-like phage; bacteriophage resistance
资金
- intramural research grant Consolidate the Foundation
Objectives: The aim of this study is to characterize a new bacteriophage able to infect Enterococcus faecalis , and to evaluate its ability to disrupt biofilm. Methods: The vB_EfaH_EF1TV (EF1TV) host -range was determined by spot test and efficiency of plating using a collection of 15 E. faecalis clinical strains. The phage genome was sequenced with a next generation sequencing approach. Anti-biofilm activity was tested by crystal violet method and confocal laser scanning microscopy. Phage-resistant mutants were selected and sequenced to investigate receptors exploited by phage for infection. Results: EF1TV is a newly discovered E. faecalis phage which belongs to the Herelleviridae family. EF1TV, whose genome is 98% identical to w EF24C, is characterized by a linear dsDNA genome of 143,507 bp with direct terminal repeats of 1,911 bp. The phage is able to infect E. faecalis and shows also the ability to degrade biofilm produced by strains of this species. The results were confirmed by confocal laser scanning microscopy analyzing the biofilm reduction in the same optical field before and after phage infection. Conclusions: The EF1TV phage shows promising features such as an obligatory lytic nature, an anti- biofilm activity and the absence of integration -related proteins, antibiotic resistance determinants and virulence factors, and therefore could be a promising tool for therapeutic applications. (C) 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据