期刊
JOURNAL OF BIOLOGICAL ENGINEERING
卷 13, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13036-019-0221-0
关键词
Microdialysis; Patch clamp; ATP; Online real-time analysis; Acupoint
资金
- Project of National Natural Science Foundation of China [8157150277]
- National Basic Research Program of China [2012CB518502]
This paper introduces a new technique combining microdialysis with patch clamp to detect the changes in ATP (adenosine triphosphate) concentration in acupoints during acupuncture. The microdialysis probe was implanted into the Zusanli acupoint (ST 36) of adult SD (Sprague Dawley) rats to sample acupoint fluid containing ATP released during acupuncture. Then, the fluid with ATP was delivered in real time to 293 T cells overexpressing P2X3 receptors, with which we could carry out patch clamp experiments. The results showed that changes in membrane currents could reflect changes in the concentration of ATP. Thus, we can successfully detect ATP released in acupoints during acupuncture in real time. This technique provides us with a new way to study the mechanism of acupuncture signal initiation.
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