AChE mRNA expression as a possible novel biomarker for the diagnosis of coronary artery disease and Alzheimer's disease, and its association with oxidative stress

Oxidative metabolic reactions and their by products have played a role in coronary artery disease (CAD) and Alzheimer's disease (AD) pathogenesis. This study was carried out on 28 patients with AD, 21 patients with CAD, and 28 healthy as control. Oxidative stress biomarkers and acetylcholinesterase (AChE) activity were assayed in plasma. mRNA expression of AChE was investigated in leukocytes of patients with CAD and AD. Thus, Alzheimer's and coronary artery patients were observed that the protein carbonyl levels and mRNA expression of AChE were increased (p<.05, p<.01, respectively). The plasma total thiol levels were decreased compared to the control group (p<.05). There was a significant relationship between amyloid beta (A beta) accumulation and oxidative stress, cholinergic gene expression. AChE gene expression and protein oxidation were increased in patients with AD and CAD. These results suggest that increased release of AChE from cells produces neurotoxic beta-amyloid plaques and may cause neurodegenerative diseases.

Automated summary

Oxidative metabolic reactions and their by-products play a role in the pathogenesis of coronary artery disease (CAD) and Alzheimer's disease (AD). This study found that levels of protein oxidation and mRNA expression of acetylcholinesterase (AChE) were increased in patients with AD and CAD. Plasma total thiol levels were decreased in these patients compared to the control group. The results suggest that increased AChE release contributes to the formation of neurotoxic beta-amyloid plaques, leading to neurodegenerative diseases.