期刊
CHEMISTRYOPEN
卷 8, 期 11, 页码 1350-1354出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/open.201900299
关键词
fluorescence; enzyme assay; host-guest chemistry; kinase; phosphatase
The combination of the macrocyclic hosts p-sulfonatocalix[4]arene and cucurbit[7]uril with the fluorescent dyes lucigenin and berberine affords two label-free enzyme assays for the detection of kinase and phosphatase activity by fluorescence monitoring. In contrast to established assays, no substrate labeling is required. Since phosphorylation is one of the most important regulatory mechanisms in biological signal transduction, the assays should be useful for identification of inhibitors and activators in high-throughput screening (HTS) format for drug discovery.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据