期刊
PLOS GENETICS
卷 15, 期 10, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1008419
关键词
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资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
- BBSRC GCRF Accelerator Award [BB/GCRF-IAA/18]
- Research England [QR 2018.19]
- University of Bath-FAPESP Sprint Award [VB-BB3FNB, FAPESP 2018/22040-8]
- FAPESP [2015/12415-6, BEPE 2017/02464-5]
- BBSRC Future Leader Fellowship [BB/N011686/1]
- BBSRC [BB/N011686/2, BB/N011686/1] Funding Source: UKRI
Microorganisms sense environmental fluctuations in nutrients and light, coordinating their growth and development accordingly. Despite their critical roles in fungi, only a few G-protein coupled receptors (GPCRs) have been characterized. The Aspergillus nidulans genome encodes 86 putative GPCRs. Here, we characterise a carbon starvation-induced GPCR-mediated glucose sensing mechanism in A. nidulans. This includes two class V (gprH and gprI) and one class VII (gprM) GPCRs, which in response to glucose promote cAMP signalling, germination and hyphal growth, while negatively regulating sexual development in a light-dependent manner. We demonstrate that GprH regulates sexual development via influencing VeA activity, a key light-dependent regulator of fungal morphogenesis and secondary metabolism. We show that GprH and GprM are light-independent negative regulators of sterigmatocystin biosynthesis. Additionally, we reveal the epistatic interactions between the three GPCRs in regulating sexual development and sterigmatocystin production. In conclusion, GprH, GprM and GprI constitute a novel carbon starvation-induced glucose sensing mechanism that functions upstream of cAMP-PKA signalling to regulate fungal development and mycotoxin production.
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