期刊
SCIENTIFIC REPORTS
卷 9, 期 -, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-019-53608-1
关键词
-
资金
- Sao Paulo Research Foundation (FAPESP) [2015/50590-4, 2013/03061-0, 2015/07008-2, 2013/19486-0, 2016/0795-7, 2015/06971-3, 2016/09950-0, 2014/04105-4, 14/04105-4, 17/16089-1]
- National Council for Scientific and Technological Development (CNPq) [305748/2017-3, 428527/2018-3, 304103/2013-6, 159488/2014-1]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [14/04105-4] Funding Source: FAPESP
The repertoire of redox-active enzymes produced by the marine fungus Peniophora sp. CBMAI 1063, a laccase hyper-producer strain, was characterized by omics analyses. The genome revealed 309 Carbohydrate-Active Enzymes (CAZymes) genes, including 48 predicted genes related to the modification and degradation of lignin, whith 303 being transcribed under cultivation in optimized saline conditions for laccase production. The secretome confirmed that the fungus can produce a versatile ligninolytic enzyme cocktail. It secretes 56 CAZymes, including 11 oxidative enzymes classified as members of auxiliary activity families (AAs), comprising two laccases, Pnh_Lac1 and Pnh_Lac2, the first is the major secretory protein of the fungi. The Pnh_Lac1-mediator system was able to promote the depolymerization of lignin fragments and polymeric lignin removal from pretreated sugarcane bagasse, confirming viability of this fungus enzymatic system for lignocellulose-based bioproducts applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据