期刊
BIOTECHNOLOGY PROGRESS
卷 32, 期 6, 页码 1412-1417出版社
WILEY-BLACKWELL
DOI: 10.1002/btpr.2369
关键词
yersiniabactin; siderophore; E. coli; metabolic engineering; polyketide
资金
- NYSP2I
- SUNY 4E
- NSF [1550378]
- Directorate For Engineering
- Div Of Industrial Innovation & Partnersh [1550378] Funding Source: National Science Foundation
Biosynthesis of complex natural products like polyketides and nonribosomal peptides using Escherichia coli as a heterologous host provides an opportunity to access these molecules. The value in doing so stems from the fact that many compounds hold some therapeutic or other beneficial property and their original production hosts are intractable for a variety of reasons. In this work, metabolic engineering and induction variable optimization were used to increase production of the polyketide-nonribosomal peptide compound yersiniabactin, a siderophore that has been utilized to selectively remove metals from various solid and aqueous samples. Specifically, several precursor substrate support pathways were altered through gene expression and exogenous supplementation in order to boost production of the final compound. The gene expression induction process was also analyzed to identify the temperatures and inducer concentrations resulting in highest final production levels. When combined, yersiniabactin production was extended to similar to 175 mg L-1. (C) 2016 American Institute of Chemical Engineers
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