期刊
JOURNAL OF FUNCTIONAL FOODS
卷 64, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jff.2019.103706
关键词
Cyclocarya paliurus; Polysaccharide; Sulfation; Immunomodulatory; Spleen lymphocytes
资金
- National Natural Science Foundation of China [31471702]
- Natural Science Foundation of Jiangxi province, China [20171BCB23022]
- Graduate Innovative Special Fund Projects of Jiangxi Province, China [YC2019-B026]
Two sulfated polysaccharides SCP3 and SCPS with degree of substitution (DS) 0.45 and 0.13 were obtained by modifying the Cyclocarya paliurus polysaccharide (CP) with chlorosulfonic acid-pyridine method. Results demonstrated that the physicochemical properties, molar ratios of monosaccharide composition, molecular weights, and in vitro immunomodulatory activity were changed after being sulfated. Splenocytes proliferation assay showed that both CP and sulfated poly-saccharides have immunomodulatory, while sulfated polysaccharides were stronger. CP mainly stimulate the production of the Th1-type cytokines, SCP3 and SCP5 not only significantly enhanced the secretion level of Th1-type cytokines, but also promote the Th2-type cytokines. Sulfated polysaccharides could significantly stimulate splenic lymphocyte proliferation synergistically with ConA or LPS. SCP3 significantly promoted the proliferation of T lymphocytes, while SCP5 stimulated the proliferation of B lymphocytes, which might be related to the DS. These results indicated that sulfation could improve the immune-enhancing effects of CP and different DS has different immunomodulatory activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据