期刊
JOURNAL OF FUNCTIONAL FOODS
卷 61, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jff.2019.103501
关键词
Amentoflavone; Hedgehog signaling; Gli1; Breast cancer stem cells; Tumorsphere
资金
- National Research Foundation of Korea (NRF) [NRF-2017R1A2B4010685]
- Bio-Synergy Research Project of the Ministry of Science, ICT, and Future Planning [NRF2013M3A9C4078156]
A novel anticancer approach is to target cancer stem cells (CSCs), a rare subpopulation potentially associated with metastasis, resistance to chemotherapy, and recurrence of cancers. This study aimed to investigate the effects of amentoflavone, a natural compound identified from Chamaecyparis obtusa, on sternness of basal-like SUM159 breast CSCs, and its underlying mechanism of action. We found that amentoflavone suppressed tumorsphere formation and regulated the expression of the stem cell markers CD44, CD24, and ALDH1 in a dose dependent manner, and inhibited Hedgehog (Hh) signaling by down-regulating Smo, Gli1, OCT4, and NANOG. Knock-down of Gli1 by siRNA significantly affected the expression of CD44, CD24, ALDH1, OCT4, and NANOG, and reduced tumorsphere formation efficiency in SUM159 breast CSCs. Our findings indicate that amentoflavone inhibits SUM159 breast cancer sternness by suppressing Hh/Gli1 signaling, and is a potent agent for targeting breast CSCs.
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