期刊
BIOTECHNOLOGY JOURNAL
卷 11, 期 10, 页码 1244-1261出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201500617
关键词
Chlorella; Genetic engineering; Heterologous proteins; Microalgae; Transformation
资金
- 985 Project of Peking University
- 863 Plan of Ministry of Science and Technology of China [2012AA023107]
- National Natural Science Foundation of China [31471717, 31571807]
The species of Chlorella represent a highly specialized group of green microalgae that can produce high levels of protein. Many Chlorella strains can grow rapidly and achieve high cell density under controlled conditions and are thus considered to be promising protein sources. Many advances in the genetic engineering of Chlorella have occurred in recent years, with significant developments in successful expression of heterologous proteins for various applications. Nevertheless, a lot of obstacles remain to be addressed, and a sophisticated and stable Chlorella expression system has yet to emerge. This review provides a brief summary of current knowledge on Chlorella and an overview of recent progress in the genetic engineering of Chlorella, and highlights the advances in the development of a genetic toolbox of Chlorella for heterologous protein expression. Research directions to further exploit the Chlorella expression system with respect to both challenges and perspectives are also discussed. This paper serves as a comprehensive literature review for the Chlorella community and will provide valuable insights into future exploration of Chlorella as a promising host for heterologous protein expression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据