Microbial synthesis of poly-γ-glutamic acid: current progress, challenges, and future perspectives

Poly-gamma-glutamic acid (gamma-PGA) is a naturally occurring biopolymer made from repeating units of L-glutamic acid, D-glutamic acid, or both. Since some bacteria are capable of vigorous gamma-PGA biosynthesis from renewable biomass, gamma-PGA is considered a promising bio-based chemical and is already widely used in the food, medical, and wastewater industries due to its biodegradable, non-toxic, and non-immunogenic properties. In this review, we consider the properties, biosynthetic pathway, production strategies, and applications of gamma-PGA. Microbial biosynthesis of gamma-PGA and the molecular mechanisms regulating production are covered in particular detail. Genetic engineering and optimization of the growth medium, process control, and downstream processing have proved to be effective strategies for lowering the cost of production, as well as manipulating the molecular mass and conformational/enantiomeric properties that facilitate screening of competitive gamma-PGA producers. Finally, future prospects of microbial gamma-PGA production are discussed in light of recent progress, challenges, and trends in this field.