期刊
VIRUS RESEARCH
卷 273, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.virusres.2019.197759
关键词
Bacteriophage; Salmonella; Lipopolysaccharide; Gene expression; Virulence
类别
资金
- National Natural Science Foundation of China [31571887]
- Anhui Provincial Natural Science Foundation [1808085MC73]
- Natural Science Foundation of Anhui Higher Education Institutions [KJ2018A0314]
- Doctor's Research Foundation of Anhui Normal University [2018XJJ53]
- Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality, and Safety Control
Bacteriophages have key roles in regulating bacterial populations in most habitats. A Salmonella Typhimurium mutant (N18) with impaired sensitivity to phage fmb-p1 was obtained and examined, the adsorption efficiency of fmb-p1 to N18 was reduced to 6%, compared to more than 97% for wild type S. Typhimurium CMCC50115. Reduced adsorption was accompanied by a reduction of 90% in the LPS content compared to wild type. Electron microscopy showed phage scattered around N18 with minimal engagement, while the phage were efficiently adsorbed to the wild type with tails oriented towards the bacterial surface. Evidence suggests fmb-p1 can slightly infect N18 and this does not give rise to an increase of phage titer. RT-qPCR data show that several Salmonella genes involved in lipopolysaccharide synthesis and five virulence related genes were down-regulated upon exposure of N18 to phage fmb-p1. In contrast, phage resistance related genes such as the SOS response, restriction-modification (RM), and Cas1 gene were up-regulated in N18. These data suggest that although inefficient adsorption and entry is the primary mechanism of resistance, transcriptional responses to phage exposure indicate that alternative resistance mechanisms against phage infection are also brought to bear, including digestion of phage nucleic acids and activation of the SOS. These findings may help develop strategies for biocontrol of Salmonella where multi-resistant bacteria are encountered or emerge in applications for food production, bioremediation or wastewater treatment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据