期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 113, 期 7, 页码 1460-1467出版社
WILEY-BLACKWELL
DOI: 10.1002/bit.25926
关键词
glucose-xylose co-fermentation; sugar transporter; specificity; protein engineering
资金
- Energy Biosciences Institute [OO7G21]
Efficient glucose-xylose co-utilization is critical for economical biofuel production from lignocellulosic biomass. To enable glucose-xylose co-utilization, a highly active xylose specific transporter without glucose inhibition is desirable. However, our understanding of the structure-activity/specificity relationship of sugar transporters in general is limited, which hinders our ability to engineer xylose-specific transporters. In this study, via homology modeling and analysis of hexose sugar transporter HXT14 mutants, we identified a highly conserved YYX(T/P) motif that plays an important role in controlling the activity and specificity of sugar transporters. We demonstrated that mutating the two tyrosine residues of the motif to phenylalanine, respectively, improved glucose transport capacity across several different sugar transporters. Furthermore, we illustrated that by engineering the fourth position in the YYX(T/P) motif, the sugar specificity of transporters was significantly altered or even reversed towards xylose. Finally, using the engineered sugar transporter, genuine glucose-xylose co-fermentation was achieved. Biotechnol. Bioeng. 2016;113: 1460-1467. (c) 2016 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据