期刊
VACCINE
卷 38, 期 3, 页码 416-422出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2019.11.006
关键词
Avian influenza; H7 subtype; Baculovirus; Pandemic flu; Influenza vaccine; Conserved peptides; Flagellin; Cross-protection
资金
- company Alternative Gene Expression SL (ALGENEX) [191008-SER-IRTA-0001-AGR]
- Spanish Government, Ministerio de Economia y Competitividad de Espana (MINECO) [AGL2013-48923-C2-2-R]
- Ministry of Education, Science and Technological Development of the Republic of Serbia [173001]
- European Comission (EC) under Horizon 2020 [Transvac2-730964-INFRAIA-2016-1]
- CERCA Programme/Generalitat de Catalunya
The immunization of poultry where H5 and H7 influenza viruses (IVs) are endemic is one of the strategies to prevent unexpected zoonoses. Our group has been focused on conserved HA-epitopes as potential vaccine candidates to obtain multivalent immune responses against distinct IV subtypes. In this study, two conserved epitopes (NG-34 and CS-17) fused to flagellin were produced in a Baculovirus platform based on Trichoplusia ni larvae as living biofactories. Soluble extracts obtained from larvae expressing flagellin-NG34/CS17 antigen were used to immunize chickens and the efficacy of the vaccine was evaluated against a heterologous H7N1 HPAIV challenge in chickens. The flagellin-NG34/CSI7 vaccine protected the vaccinated chickens and blocked viral shedding orally and cloacally. Furthermore, no apparent clinical signs were monitored in 10/12 vaccinated individuals. The mechanism of protection conferred is under investigation. (C) 2019 The Authors. Published by Elsevier Ltd.
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