期刊
BIOTECHNOLOGY ADVANCES
卷 34, 期 8, 页码 1427-1441出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2016.11.002
关键词
3D tumor spheroids; Cancer therapy; In vitro models; Methodologies; Therapeutic screening
资金
- FEDER funds through the POCI - COMPETE - Operational Programme Competitiveness and Internationalisation in Axis I - Strengthening research, technological development and innovation [POCI-01-0145-FEDER-007491]
- National Funds by FCT - Foundation for Science and Technology [UID/Multi/00709/2013]
- [SFRH/BD/103507/2014]
- [SFRH/BD/109482/2015]
- [SFRH/BD/103506/2014]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/103506/2014, SFRH/BD/103507/2014] Funding Source: FCT
In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeutics, both at a small and large scale. Nevertheless, few reports have focused on describing the tools and techniques currently available to extract significant biological data from these models. Such information will be fundamental to drug and therapeutic discovery process using 3D cell culture models. The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids. (C) 2016 Elsevier Inc All rights reserved.
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