期刊
TRANSBOUNDARY AND EMERGING DISEASES
卷 67, 期 2, 页码 865-876出版社
WILEY-HINDAWI
DOI: 10.1111/tbed.13410
关键词
astrovirus; genetic distance; gosling gout; mutation analysis; phylogenetic tree
资金
- National Natural Science Foundation of China [31802185, 31870917]
- Scientific and Technological Project of Henan Province [182107000040]
- Key Scientific and Technological Project of the Education Department of Henan province [18A230012]
- University of Henan Province [20IRTSTHN024]
- Key Scientific and Technological Project of Nanyang City [KJGG2018144, KJGG2018069]
- Technological Project of Nanyang normal university [18046, 2019QN009, 2019CX001]
Since February 2017, severe outbreaks of fatal gout caused by novel gosling astroviruses (GoAstVs) have occurred in several Chinese provinces, causing a considerable economic impact on the poultry industry. To assess the infection status of GoAstVs causing gout, 165 clinical samples were collected from goslings from seven farms located in different Chinese provinces, and they were screened for viral infection. Seven GoAstV strains were completely sequenced. The positive infection rates of GoAstV, goose parvovirus, reovirus, goose haemorrhagic polyomavirus and Tembusu virus were 100%, 9.69%, 3.64%, 0% and 0%, respectively, indicating the role of GoAstV in gout. The genomes of all seven GoAstV strains were 7170-nt long and encoded three open reading frames (ORFs), namely, ORF1a, ORF1b and ORF2. Sequence and phylogenetic analyses of the seven GoAstV strains showed that these were avastroviruses and were closely related to viruses classified within Avastrovirus 3 and turkey astrovirus 2. Moreover, the mutation rates of ORF1a and ORF2 were high, and ORF1a was highly mutated at amino acid loci 545-580. The tertiary structure of the mutated ORF2 protein was smooth, and its antigenic epitope was highly mutated, which may be related to the pathogenicity of the virus and caused by antibody pressure from the host. These findings enrich our understanding of the evolution of novel GoAstVs causing gout and their circulation as well as lay the foundation for the selection of vaccine strains.
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