期刊
TOXICOLOGY LETTERS
卷 315, 期 -, 页码 47-54出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2019.08.015
关键词
Particulate matter; Surfactant protein A; Lamellar bodies; Reactive oxygen species; N-acetylcysteine
类别
资金
- National Natural Science Foundation of China [31670742, 81302246]
- Guangdong Natural Science Foundation [2015A030313521, 2018A030307043]
Particulate matter with a diameter of less than 2.5 mu m (PM2.5) easily deposits on lung alveoli and degrades human health. Surfactant protein A (SP-A) is the most abundant pulmonary surfactant protein stored in lamellar bodies (LBs) of alveolar epithelial type II cells. The impacts of PM2.5 on SP-A are multifaceted and intractable, and the underlying mechanism remains unclear. In this study, the expression and distribution of SP-A in Balb/c mice and A549 cells under PM2.5 exposure were investigated. The results showed that the low and medium concentration of PM2.5 gradually enhanced SP-A protein and mRNA expression, whereas the high concentration of PM2.5 conspicuously decreased SP-A protein but not its mRNA compared with the control. The trafficking of SP-A to LBs was gradually disturbed, and concomitantly, the lesions of LBs responsible for the transport and storage of SP-A protein were exacerbated with increased PM2.5 concentration. Reactive oxygen species production abundantly increased upon PM2.5 exposure, and it was antagonized by the oxidant inhibitor N-acetylcysteine. Subsequently, the injured LBs and the decrease in SP-A expression under exposure to the high concentration of PM2.5 were well rescued. The present study provides a new perspective to investigate the adverse effects of PM2.5 or diesel exhaust particles on other proteins transported to and stored in LBs.
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