期刊
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
卷 30, 期 3, 页码 607-613出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/13102818.2016.1150792
关键词
Electroporation; MOSFET; crowbar; electropermeabilization; nanosecond pulse; electric field
In this work, we present a novel electroporator which is capable of generating single and bursts of high power (3 kV, 60 A) square wave pulses of variable duration (100 ns to 1 ms) with predefined repetition frequency (1 Hz to 3.5 MHz). The proposed synchronized crowbar implementation ensures a constant pulse rise and fall times, which are independent from the load, thus highly relevant in electroporation. The electroporator was successfully tested for the inactivation of the human pathogen Candida albicans. The device is compatible with standard commercial electroporation cuvettes.
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