期刊
THYROID
卷 30, 期 1, 页码 116-132出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/thy.2019.0175
关键词
thyroid hormone receptor beta; breast cancer stem cells; self-renewal; estrogen receptors
资金
- CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI)
- MINECO, AEI, FEDER, UE [BFU2014-53610-P]
- Spanish Ministry of Economy and Competitiveness [CIBERONC CB/16/00228, SAF2017-90604-REDT]
- Comunidad de Madrid [B2017/BMD-3724]
- FEDER funds
- NATIONAL CANCER INSTITUTE [ZIABC008752, ZIABC011191] Funding Source: NIH RePORTER
Background: A subpopulation of cancer stem cells (CSCs) with capacity for self-renewal is believed to drive initiation, progression, and relapse of breast tumors. Methods: Since the thyroid hormone receptor beta (TR beta) appears to suppress breast tumor growth and metastasis, we have analyzed the possibility that TR beta could affect the CSC population using MCF-7 cells grown under adherent conditions or as mammospheres, as well as inoculation into immunodeficient mice. Results: Treatment of TR beta-expressing MCF-7 cells (MCF7-TR beta cells) with the thyroid hormone triiodothyronine (T3) decreased significantly CD44(+)/CD24(-) and ALDH(+) cell subpopulations, the efficiency of mammosphere formation, the self-renewal capacity of CSCs in limiting dilution assays, the expression of the pluripotency factors in the mammospheres, and tumor initiating capacity in immunodeficient mice, indicating that the hormone reduces the CSC population present within the bulk MCF7-TR beta cultures. T3 also decreased migration and invasion, a hallmark of CSCs. Transcriptome analysis showed downregulation of the estrogen receptor alpha (ER alpha) and ER-responsive genes by T3. Furthermore, among the T3-repressed genes, there was an enrichment in genes containing binding sites for transcription factors that are key determinants of luminal-type breast cancers and are required for ER binding to chromatin. Conclusion: We demonstrate a novel role of TR beta in the biology of CSCs that may be related to its action as a tumor suppressor in breast cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据