Influence of Sexcel™ (gender ablation technology) gender-ablated semen in fixed-time artificial insemination of beef cows and heifers

Previous research has indicated that gender-sorted semen has decreased conception rates compared to conventional semen. A new method to skew the gender ratio of semen has been developed that does not use traditional sorting technology. Thus, the objective of this study was to evaluate the use of genderablated semen in a fixed-time Al protocol. Beef heifers and cows (n = 878) from six herds were synchronized with the 7-d CO-Synch plus CIDR protocol, and artificially inseminated (Al) after CIDR removal (cows 60-66 h; heifers 52-56 h). Estrus detection aids were applied at CIDR removal and estrus activity was determined at time of Al. Animals remained separated from bulls for at least 10 d after Al. Pregnancy success and fetal age were determined between d 28 and 70 after Al. Semen from five sires was utilized in this study, with two sires used in each herd. Sire 1 was used in all herds and the use of the second sire varied by herd (both conventional and gender-ablated semen from each sire was used in each herd). Data were analyzed using the GLIMMIX procedure in SAS and included the influence of semen type, estrus expression, semen type by estrus expression, dam age, and sire in the model. Herd was included as a random variable. Overall, conventional semen had greater conception rates compared to gender-ablated semen (P < 0.01; 67% vs 52%), and cows that had fully activated patches and partially activated patches had greater conception rates compared to animals that had not exhibited estrus (P < 0.01; 69%, 65%, and 45%). There was no semen type by estrus expression interaction (P = 0.24). Conception rates tended to be greater for conventional semen among animals that had activated patches (P = 0.06; 73% vs 65%) and partially activated patches (P = 0.06; 72% vs 59%). Conception rates were greater for conventional semen (P < 0.01; 56% vs 33%) among animals that did not exhibit estrus. There was no effect of dam age (P = 0.40) or sire (P = 0.92) on conception rates. In conclusion, Sexcellid gender-ablated semen can successfully be used in fixed-time Al protocols among animals that exhibit estrus, but caution should be used among animals that do not exhibit estrus. (C) 2019 The Authors. Published by Elsevier Inc.