期刊
BIOTECHNIQUES
卷 61, 期 4, 页码 191-201出版社
BIOTECHNIQUES OFFICE
DOI: 10.2144/000114459
关键词
colony array; yeast; bacteria; synthetic genetic array; microorganism; large-scale screen
资金
- Czech Science Foundation [P305/12/P040]
- Charles University in Prague [UNCE 204013]
- Commonwealth Scientific and Industrial Research Organisation of Australia
- BBSRC [BB/I012451/1]
- Wellcome Trust [095598/Z/11/Z]
- BBSRC [BB/I012451/1] Funding Source: UKRI
Microbial colony growth can serve as a useful readout in assays for studying complex genetic interactions or the effects of chemical compounds. Although computational tools for acquiring quantitative measurements of microbial colonies have been developed, their utility can be compromised by inflexible input image requirements, non-trivial installation procedures, or complicated operation. Here, we present the Spotsizer software tool for automated colony size measurements in images of robotically arrayed microbial colonies. Spotsizer features a convenient graphical user interface (GUI), has both single-image and batch-processing capabilities, and works with multiple input image formats and different colony grid types. We demonstrate how Spotsizer can be used for high-throughput quantitative analysis of fission yeast growth. The user-friendly Spotsizer tool provides rapid, accurate, and robust quantitative analyses of microbial growth in a high-throughput format. Spotsizer is freely available at https://data.csiro.au/dap/landingpage?pid=csiro:15330 under a proprietary CSIRO license.
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