期刊
TECHNICAL PHYSICS LETTERS
卷 45, 期 11, 页码 1140-1143出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1063785019110191
关键词
holographic microscopy; holographic tomography; morphological characteristics of cells; cell segmentation
资金
- Council on grants of the President of the Russian Federation for Support of Young Scientists [SP-2349.2019.4]
Algorithms of cell segmentation on two-dimensional phase images and three-dimensional distributions of a refractive index obtained by means of digital holographic microscopy and tomography are developed. The proposed algorithms are optimized for determining cell morphology characteristics including the cell volume, projection area, and surface area. A comparative analysis of the error of cell volume determination by holographic methods using the proposed cell segmentation algorithms and the standard method of confocal fluorescence microscopy has been performed.
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