期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 229, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117875
关键词
Gold nanocluster; Fluorescence; Alkaline phosphatase; Human osteosarcoma cells; Enzyme inhibitor
类别
资金
- National Natural Science Foundation of China [21675024, 21804021]
- Program for Innovative Leading Talents in Fujian Province [2016B016]
- Natural Science Foundation of Fujian Province [2019J01304]
- Science and Technology Project of Fujian Province [2018L3008]
Gold nanoclusters (AuNCs) have attracted much attention as signal transducers in photoluminescence chemical/biological sensors. Herein, we employ bovine serum albumin/3-mercaptopropionic acid co-modified AuNCs as a fluorescence probe, Fe3+ as a quencher, and pyrophosphate as an alkaline phosphatase (ALP) substrate and Fe3+ chelator to design a novel biosensor for ALP detection, achieving a detection linear range of 0.8-16 U/L and a detection limit of 0.78 U/L. The developed method is successfully applied to the detection of ALP in human osteosarcoma cells and is shown to be suited for ALP inhibitor screening. (c) 2019 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据