期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 228, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117764
关键词
Surface-enhanced Raman scattering; Molecular imprinting; Lysozyme; Protein; Density functional theory
类别
资金
- National Natural Science Foundation of China [51579057, 51779065]
- State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology [2019DX11]
Surface-enhanced Raman scattering (SERS) is a powerful bioanalytical technique that opens opportunities for early disease diagnosis and treatment by detecting biomarkers. However, the low sensitivity, selectivity, and reproducibility in the bioanalytical SERS are the main obstacles for clinical use. Herein, we demonstrate a high sensitive and selective label-free lysozyme biomarker detection platform based on coupling of SERS with molecular imprinting technique. The hierarchical silver microspheres with dendritic structure are controllably fabricated by a wet-chemical self-assembly approach. Based on selection of surface-active regions by density functional theory (DFT) simulations, a thin MIPs film (thickness < 15 nm) is then grafted on Ag microsphere surface through surface imprinting. As a result, the final synthesized Ag@MIPs hybrid exhibits as low as 5 ng mL(-1) detection limit for target lysozyme, and high selectivity and reproducibility. Intensive hot spots in Ag@MIPs confirmed by Raman mapping give rise to the high-performance SERS. Meanwhile, DFT calculations are employed to investigate the SERS spectra and assist the assignment for the characteristic peaks of lysozyme. We believe that the present study provides a reliable and high-sensitive protocol for label-free protein biomarkers detection. (c) 2019 Elsevier B.V. All rights reserved
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