期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 228, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117766
关键词
Dynamical cyclic behaviors; Lipid droplets; Lipophagy; Two-photon
类别
资金
- National Natural Science Foundation of China [51772002, 51432001, 51972001, 21805001]
- Changjiang Scholars and Innovative Research Team in University
- Science Research Foundation of the Higher Education Institutions of Anhui Province [KJ2019A0771, KJ2018A0024, KJ2018A0514, KJ2017A008]
- Anhui University Research Start-up Fund [S020118002/039, S020118002/089]
- Anhui Provincial Natural Science Foundation [1808085ME55]
Lipid droplets (LDs) are organelles featuring in intracellular storage of neutral lipids, which are involved with many biological processes. Monitoring the dynamical cyclic behaviors of cellular LDs poses great importance for early disease diagnosis. Herein, two coumarin-based LDs-specific fluorescence probes exhibited turn-on and two-photon fluorescence triggered by breaking their aggregation states. By virtue of establishing oil/water emulsions model to simulate LDs, the behaviors of turn-on fluorescence were elucidated, which benefited for the enhancement of selectivity of the probes to LDs. Then, we highlight a LDs-specific coumarin-based two-photon probe (L1) with high photo-stability to monitor the dynamic cyclic behaviors of LDs in cells, unraveling the changes of LDs quantity during lipophagy and the reproduction of LDs to prevent lipotoxicity. We believe the probe offers a convenient way to investigate the biology of LDs. (c) 2019 Elsevier B.V. All rights reserved.
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