期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 222, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117277
关键词
Gold and magnetic particles (GoldMag); Mimetic peroxidase; Ascorbic acid (AA); Colorimetric detection
类别
资金
- National Natural Science Foundation of China [31370649, 31201376]
- China Postdoctoral Science Foundation [2014T70304, 2013M531009]
- Heilongjiang Postdoctoral Science Foundation [LBH-Z13002]
- Natural Science Foundation of Heilongjiang Province of China [C2016034]
A new scheme for sensitive and rapid colorimetric detection of ascorbic acid (AA) has been developed by the GoldMag-ABTS free radical scavenging system. The well-dispersed Gold and magnetic particles (GoldMag) was successfully prepared by self-assembly method and characterized by Fourier transform infrared (FTIR), X-Ray Photoelectron Spectroscopic (XPS) techniques. Nanocomposites combine the advantages of superparamagnetic, biocompatibility and high catalytic activity of Fe3O4 and gold nanoparticles (AuNPs) and exhibit enhanced the intrinsic peroxidase-like activity, which can be used to catalyze the oxidation of the peroxidase substrates 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) produce a green-colored product in presence of hydrogen peroxide. Ascorbic acid as an effective antioxidant have scavenging effects on ABTS radicals and induce the reduction of green ABTS.+ to colorless ABTS(2-), resulting in a significant green color fading. On this basis, a rapid, sensitive and selective colorimetric assay for ascorbic acid has been developed. Under optimal conditions, ascorbic acid has a linear response range from 0.01mmol/L to 1 mmol/L with a detection limit of 0.12 mu mol/L and a short assay time of the 30 s. Furthermore, the colorimetric systemshowed good sensitivity, stability, selectivity, and repeatability. It also successfully applied to the determination of ascorbic acid in real samples. (C) 2019 Elsevier B.V. All rights reserved.
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