期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 222, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117230
关键词
Fluorescent sensor; Aggregation induced emission; Excited state intramolecular proton transfer; ONOO-; Living cell imaging
类别
资金
- Scientific Research Fund of Hunan Province Education Department [17B181]
- Natural Science Foundation of Hunan Province [2017JJ2195]
- National Natural Science Foundation of China [21507028]
- Open Project Program of Key Laboratory of Preparation and Application of Environmental Friendly Materials (Jilin Normal University), Ministry of Education of China [2017010]
Developing specific and sensitive method for endogenous peroxynitrite (ONOO-) in living systems is valuable to understand its various pathological events. In this work, a simple and novel fluorescent probe (HPP) based on aggregation induced emission (AIE) as well as excited state intramolecular proton transfer (ESIPT) processes for endogenous ONOO- detection was constructed containing a diphenylphosphinate as the reactive site and a salicylaldehyde azine as the fluorophore. The probe showed specific fluorescence turn-on response toward ONOO- owing to the diphenylphosphinate group of HPP reacted with ONOO-, releasing the salicylaldehyde azine with both AIE and ESIPT characteristics. In addition, the probe exhibited a large Stokes shift, an excellent light-up ratio, high selectivity and excellent sensitivity with a low detection limit of 8 x 10(-8) M for endogenous ONOO-. Moreover, the probe could be applied to bioimaging of endogenous ONOO- in live cell, which demonstrated the probe can be used as effective tool for investigation of ONOO- in biological systems. (C) 2019 Elsevier B.V. All rights reserved.
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