期刊
SMALL
卷 15, 期 45, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201902699
关键词
amino-functionalized graphene oxide; Caenorhabditis elegans; graphene oxide; innate immunity
类别
资金
- ANR [ANR-15-GRFL-0001-05]
- MIUR JTC Graphene 2015 (G-IMMUNOMICS project)
- European Union [785219]
- Centre National de la Recherche Scientique (CNRS)
- Agence Nationale de la Recherche (ANR) through the LabEx project Chemistry of Complex Systems [ANR-10-LABX-0026_CSC]
- International Center for Frontier Research in Chemistry (icFRC)
- Deutsche Forschungsgemeinschaft [SCHU 2494/3-1, SCHU 2494/7-1, SFB 829, SFB 670, KFO 286, KFO 329]
- Deutsche Krebshilfe [70112899]
- COST action [BM1408]
Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. This study describes adverse effects of GO and amino-functionalized GO (GONH(2)) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA-Sequencing data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK-1, which is a well-established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk-1 mutants show reduced tissue-functionality and facultative vivipary. In a direct comparison, GONH(2) exposure does not cause detrimental effects in the wild type or in pmk-1 mutants, and the innate immune response is considerably less pronounced. This work establishes enhanced biocompatibility of amino-functionalized GO in a whole-organism, emphasizing its potential as a biomedical nanomaterial.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据