期刊
BIOSENSORS & BIOELECTRONICS
卷 77, 期 -, 页码 725-732出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.10.049
关键词
Fluorescent probe; SO2 derivatives; Benzo[e]indolium; Mitochondria-targeted; Ratiometric; Imaging
类别
资金
- National Natural Science Foundation of China [21203138, 31371750]
- Natural Science Foundation of Tianjin [13JCQNJC05300]
- Science Foundation of China [21262050, 21302165]
- Foundation of the Department of Science and Technology of Yunnan Province of China [2014HB008]
In this study, we report a ratiometric fluorescent probe (CZBI) for sulfur dioxide (SO2) derivatives based on the conjugate of carbazole and benzo[e]indolium, which displays colorimetric and ratiometric fluorescence dual response to HSO3-. The probe can quantitatively detect HSO3- with high specificity, fast response (within 40 s) as well as low detection limit (10 nM). A 1,4-nucleophilic addition reaction was proposed for the sensing mechanism of this probe, which was confirmed by H-1 NMR and HR-MS spectra. Fluorescence co-localization studies demonstrated that CZBI was a specific mitochondria-targeted fluorescent probe for SO2 derivatives with excellent cell membrane permeability. Furthermore, fluorescence imaging of HeLa cells indicated that CZBI could be used for monitoring the intrinsically generated intracellular SO2 derivatives in living cells by ratiometric fluorescence imaging. Thus, CZBI has a great potential application for exploring the role played by SO2 derivatives in biology. (C) 2015 Elsevier B.V. All rights reserved.
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