期刊
BIOSENSORS & BIOELECTRONICS
卷 86, 期 -, 页码 985-989出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.07.045
关键词
Autonomous assembly; Dendrimer-like DNA nanostructure; Enzyme-free; Signal amplification; Colorimetric detection; Nucleic acid
类别
资金
- National Natural Science Foundation of China, China [21275104, 21377089, 21407109, 21475091]
Self-assembly of DNA nanostructures is of great importance in nanomedicine, nanotechnology and biosensing. Herein, a novel target-catalyzed autonomous assembly pathway for the formation of dendrimer-like DNA nanostructures that only employing target DNA and three hairpin DNA probes was proposed. We use the sticky-ended Y shape DNA (Y-DNA) as the assembly monomer and it was synthesized by the catalyzed hairpin assembly (CHA) instead of the DNA strand annealing method. The formed Y-DNA was equipped with three ssDNA sticky ends and two of them were predesigned to be complementary to the third one, then the dendrimer-like DNA nanostructures can be obtained via an autonomous assembly among these sticky-ended Y-DNAs. The resulting nanostructure has been successfully applied to develop an enzyme-free and signal amplified gold nanoparticle (AuNP)-based colorimetric nucleic acids assay. (C) 2016 Elsevier B.V. All rights reserved.
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