期刊
BIOSENSORS & BIOELECTRONICS
卷 86, 期 -, 页码 885-891出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.07.026
关键词
Mitochondria selective probe; Viscosity probe; Fluorescent probe; Two photon probe; Mitochondria transport in neurons
类别
资金
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2012R1A3A2048814]
- National Research Foundation (NRF) - Korean Government [2012R1A2A1A03670456]
We present here a viscosity sensitive fluorescent dye, namely thiophene dihemicyanine (TDHC), that enables the specific staining of mitochondria. In comparison to the common mitochondria tracker (Mitotracker Deep Red, MTDR), this dye demonstrated its unique ability for robust staining of mitochondria with high photostability and ultrahigh signal-to-noise ratio (SNR). Moreover, TDHC also showed high sensitivity towards mitochondria membrane potential (Delta Psi(m)) and intramitochondria viscosity change. Consequently, this dye was utilized in real-time monitoring of mitochondria transport in primary cortical neurons. Finally, the Two-Photon Microscopy (TPM) imaging ability of TDHC was also demonstrated. (C) 2016 Elsevier B.U. All rights reserved.
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