期刊
BIOSENSORS & BIOELECTRONICS
卷 80, 期 -, 页码 574-581出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.091
关键词
Electrochemical; Aptasensor; Signal amplification; Aflatoxin B1; Gold nanoparticle
类别
资金
- NSFC Grant [21475030, 31301460]
- Science and Technology Research Project of Anhui Province [15czz03109]
- National 10000 Talents Youth Top-notch Talent Program
- National and Zhejiang Public Benefit Research Project [201313010, 2014C32051]
- Jiangsu Science and Technology Support Program [BE201373, 2012780]
An electrochemical aptasensor for trace detection of aflatoxin B1 (AFB1) was developed by using an aptamer as the recognition unit while adopting the telomerase and EXO III based two-round signal amplification strategy as the signal enhancement units. The telomerase amplification was used to elongate the ssDNA probes on the surface of gold nanoparticles, by which the signal response range of the signal-off model electrochemical aptasensor could be correspondingly enlarged. Then, the EXO III amplification was used to hydrolyze the 3'-end of the dsDNA after the recognition of target AFB1, which caused the release of bounded AFB1 into the sensing system, where it participated in the next recognition-sensing cycle. With this two-round signal amplified electrochemical aptasensor, target AFB1 was successfully measured at trace concentrations with excellent detection limit of 0.6*10(-4) ppt and satisfied specificity due to the excellent affinity of the aptamer against AFB1. Based on this designed two round signal amplification strategy, both the sensing range and detection limit were greatly improved. This proposed ultrasensitive electrochemical aptasensor method was also validated by comparison with the classic instrumental methods. Importantly, this hetero-enzyme based two-round signal amplified electrochemical aptasensor offers a great promising protocol for ultrasensitive detection of AFB1 and other mycotoxins by replacing the core recognition sequence of the aptamer. (C) 2016 Elsevier B.V. All rights reserved.
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