期刊
BIOSENSORS & BIOELECTRONICS
卷 80, 期 -, 页码 525-531出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.025
关键词
beta-agonists; Aptasensor; Electrochemical detection; Selectivity
类别
资金
- Guangdong Province Agricultural Science & Technology Program [2012A020200003]
- Scientific Cultivation and Innovation Fund Program of Jinan University [21612453]
beta-agonists are phenylethanolamines with different substituent groups on the aromatic ring and the terminal amino group which have the effect of nutrition redistribution and can accumulate in body tissues causing acute or chronic poisoning when consumed. Therefore, it is very important to establish a fast screening method for the detection of several kinds of beta-agonists in food safety control. In this study, the aptamer-agonists (AP-Ago) has screened out by Isothermal Titration Calorimetric method. AP-Ago was a single-strand DNA with 22 base-pairs. The dissociation constant (K-d) to phenylethanolamine (PHL) was 3.34 x 10(-5) mol L-1. The AP-Ago based electrode was constructed by self-assembling on gold electrode. A label-free electrochemical aptasensor was then developed with AP-Ago-based gold electrode, which was sensitive to phenylethanolamine(PHL), clenbuterol (CLB), ractopamine (RAC), salbutamol (SAL) and procaterol (PRO). The detection limits were 0.04 ng/mL (RAC), 0.35 pg/mL (CLB), 1.0 pg/mL (PHL), 0.53 pg/mL (SAL) and 1.73 pg/mL(PRO), respectively, The detection time was 15 min. The reproductivity of the mentioned aptasensor is good with RSD of 2.09%. Comparing with ELISA and HPLC on beta-agonists detection in actual sample, this aptasensor is advantage of fewer steps and fast screen detection of these five beta-agonists or their mixtures. This study suggests that the aptasensor can be developed to a rapid screening means with multi-beta-agonists (may be one or more) in sample. (C) 2016 Elsevier B.V. All rights reserved.
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