期刊
BIOSENSORS & BIOELECTRONICS
卷 78, 期 -, 页码 538-546出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.003
关键词
Aptamer; Microfluidic paper-based analytical device; Equipment-free quantitative detection; Point-of-care testing; Adenosine
类别
资金
- National Natural Science Foundation of China [21105017, 21205021, 21365009, 21565012]
- Guangxi Natural Science Foundation [2014GXNSFAA118033, 2015GXNSFFA139005]
- Open Foundation of State Key Laboratory of Chemo/biosensing and Chemometrics in Hunan University, China [2013001, 2014005]
- Project of High Level Innovation Team and Outstanding Scholar in Guangxi Colleges and Universities, China
This work initially describes the design of low-cost, naked-eye quantitative aptamer-based assays by using microfluidic paper-based analytical device (PAD). Two new detection motifs are proposed for quantitative mu PAD measurement without using external electronic readers, which depend on the length of colored region in a strip-like mu PAD and the number of colorless detection microzones in a multi-zone mu PAD. The length measuring method is based on selective color change of paper from colorless to blue black via formation of iodine-starch complex. The counting method is conducted on the basis of oxidation-reduction reaction between hydrogen peroxide and potassium permanganate. Their utility is well demonstrated with sensitive, specific detection of adenosine as a model analyte with the naked eye in buffer samples and undiluted human serum. These equipment-free quantitative methods proposed thus hold great potential for the development of more aptamer-based assays that are simple, cost-efficient, portable, and user-friendly for various point-of-care applications particularly in resource-constrained environments. (C) 2015 Elsevier B.V. All rights reserved.
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