Specific and selective electrochemical immunoassay for Pseudomonas aeruginosa based on pectin-gold nano composite

In this report, we have successfully fabricated an immunosensor for detection of Pseudomonas aeruginosa in water. The monoclonal antibody was immobilized on the surface modified with CCLP (Calcium Cross Linked Pectin)-Au NPs (gold nanoparticles)/Glassy Carbon Electrode. The building of the immunosensor was evaluated in each step by cyclic voltammetry (CV) and impedance spectroscopy (EIS). The electrochemical detection was done based on the anti rabbit IgG HRP (Horseradish Peroxidase) which binds to the immune complex and the response was observed using Hydro Quininone (HQ) and Hydrogen peroxide (H2O2) in PB (Phosphate Buffer) electrolyte. From the results, the sensitivity range is from 10(1) to 10(7) CFU/ml and LOD is calculated as 9 x 10(2) CFU/ml. The developed immunosensor also have high selectivity, stability, reproducibility and reusability. (C) 2015 Elsevier B.V. All rights reserved.