期刊
BIOSENSORS & BIOELECTRONICS
卷 75, 期 -, 页码 411-419出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.07.045
关键词
N-acyl-l-homoserine lactones; Magnetic molecularly imprinted polymers; Electrochemical sensor; Fe3O4
类别
资金
- National Natural Science Foundation of China [31371768]
- Program for New Century Excellent Talents in Jiangnan University
- national center for functional food engineering
- Priority Academic Program Development of Jiangsu Higher Education Institutions
We have developed a novel and economical electrochemical sensor to measure Gram-negative bacterial quorum signaling molecules (AHLs) using magnetic nanoparticles and molecularly imprinted polymer (MIP) technology. Magnetic molecularly imprinted polymers (MMIPs) capable of selectively absorbing AHLs were successfully synthesized by surface polymerization. The particles were deposited onto a magnetic carbon paste electrode (MGCE) surface, and characterized by electrochemical measurements. Differential Pulse Voltammetry (DPV) was utilized to record the oxidative current signal that is characteristic of AHL. The detection limit of this assay was determined to be 8 x 10(-10) mol L-1 with a linear detection range of 2.5 x 10(-9) mol L-1 to 1.0 x 10(-7) mol L-1. This Fe3O4@SiO2-MIP-based electrochemical sensor is a valuable new tool that allows quantitative measurement of Gram-negative bacterial quorum signaling molecules. It has potential applications in the fields of clinical diagnosis or food analysis with real-time detection capability, high specificity, excellent reproducibility, and good stability. (C) 2015 Elsevier B.V. All rights reserved.
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