期刊
BIOSENSORS & BIOELECTRONICS
卷 79, 期 -, 页码 449-456出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.12.061
关键词
Porous Pt-Pd; Superoxide dismutase; Ultrahigh sensitivity; Superoxide anion detection; O-2(center dot-) with trace level; Living cells
类别
资金
- National Natural Science Foundation of China (NSFC) [21305044]
- Science and Technology Commission of Shanghai Municipality (STCSM) [13510710900]
Considering the critical roles of superoxide anion (O-2(center dot-)) in pathological conditions, it is of great urgency to establish a reliable and durable approach for real-time determination of O-2(center dot-). In this study, we propose a porous Pt-Pd decorated superoxide dismutase (SOD) sensor for qualitative and quantitative detection O-2(center dot-). The developed biosensor exhibits a fast, selective and linear amperometric response upon O-2(center dot-) in the concentration scope of 16 to 1536 mu M (R-2=0.9941), with a detection limit of 0.13 mu M (S/N=3) and a low Michaelis-Menten constant of 1.37 mu M which indicating a high enzymatic activity and affinity to O-2(center dot-). Inspiringly, the proposed sensor possesses an ultrahigh sensitivity of 1270 mu A mM(-1) cm(-2). In addition, SOD/porous Pt-Pd sensor exhibits excellent anti-interference property, reproducibility and long-term storage stability. Beyond our expectation, the trace level of O-2(center dot-)-released from living cells has also been successfully captured. These satisfactory results are mainly ascribed to (1) the porous interface with larger surface area and more active sites to provide a biocompatible environment for SOD (2) the specific biocatalysis of SOD towards O-2(center dot-)-and (3) porous Pt-Pd nanomaterials fastening the electron transfer. The superior electrochemical performance makes SOD/porous Pt-Pd sensor a promising candidate for monitoring the dynamic changes of O-2(center dot-)-in vivo. (C) 2015 Elsevier B.V. All rights reserved.
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