期刊
BIOSENSORS & BIOELECTRONICS
卷 86, 期 -, 页码 270-276出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.06.054
关键词
Facile synthesis; Copper nanoclusters; Boracic acid functionalization; Glycoproteins
类别
资金
- National Natural Science Foundation of China [21375095, 20975054]
- Program for Innovative Research Team in University of Tianjin [TD12-5038]
- Program for Young Backbone Talents in Tianjin [ZX110GG015, ZX110185]
- Doctor Foundation of Tianjin Normal University [52XB1510]
As an emerging class of fluorescent probes, copper nanoclusters (Cu NCs) have been considered as an intriguing candidate for detecting biomoleculars due to their outstanding fluorescent properties, excellent biocompatibility and low cost. Herein, we fabricated bovine serum albumin (BSA) protected Cu NCs (BSA-Cu NCs) and further functionalized them with 3-aminophenylboronic acid (APBA) for selectively discerning glycoproteins. In aqueous solution, Cult ions were directly reduced into BSA-Cu NCs by hydrazine hydrate (N2H4 . H2O) at room-temperature using BSA as the capping agent. The synthetic process was very rapid, simple and easy for controlling due to the lack of any other complicated procedure such as heating and adjusting the pH value of the reactive mixture. The APBA-Cu NCs showed strong fluorescent emission at 630 nm in the red range. So it can effectively avoid the disturbance of auto-fluorescence in biosamples. The fluorescence of the APBA-Cu NCs was obviously quenched by glycoprotein samples. Then, the APBA-Cu NCs were employed as a probe for selective capture and sensitive detection of glycoproteins with a wide linear range of 5-220 nM and a low detection limit of 2.60 nM owing to the covalent reaction between the boric acid group of APBA and the cis-glycol groups of the glycoproteins. The developed method was also successfully applied to determine glycoproteins in egg white of chickens and human urine samples with quantitative spike recoveries from 95% to 104%. (C) 2016 Elsevier B.V. All rights reserved.
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