期刊
BIOSENSORS & BIOELECTRONICS
卷 81, 期 -, 页码 159-165出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.02.061
关键词
Surface plasmon resonance; Aflatoxin; Polymer brushes; Gold nanoparticles; Inhibition immunoassay; Signal amplification
类别
资金
- Initial Training Network, SA-MOSS - FP7 Marie Curie Actions of the European Commission (FP7-PEOPLE-ITN)
- Grant Agency of the Czech Republic (GACR) [15-09368Y]
The rapid and sensitive detection of aflatoxin M-1 (AFM(1)) in milk by using surface plasmon resonance (SPR) biosensor is reported. This low molecular weight mycotoxin is analyzed using an indirect competitive immunoassay that is amplified by secondary antibodies conjugated with Au nanoparticles. In order to prevent fouling on the sensor surface by the constituents present in analyzed milk samples, an interface with poly(2-hydroxyethyl methacrylate) p(HEMA) brush was employed. The study presents a comparison of performance characteristics of p(HEMA)-based sensor with a regularly used polyethylene glycol -based architecture relying on mixed thiol self-assembled monolayer. Both sensors are characterized in terms of surface mass density of immobilized AFM(1) conjugate as well as affinity bound primary and secondary antibodies. The efficiency of the amplification strategy based on Au nanoparticle is discussed. The biosensor allowed for highly sensitive detection of AFM(1) in milk with a limit of detection (LOD) as low as 18 pg mL(-1) with the analysis time of 55 min. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据