期刊
BIOSENSORS & BIOELECTRONICS
卷 86, 期 -, 页码 530-535出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.07.021
关键词
Ratiometric fluorescent probe; Test paper; Colorimetric quantification; Blood glucose
类别
资金
- National Natural Science Foundation of China [21275075, 21335006, 21475135, 21375131, 21275145]
- National Basic Research Program of China [2015CB932002]
- China-Singapore Joint Project [2015DFG92510]
- Science and Technology Service Network Initiative [KFJ-SW-STS-172]
- Natural Science Foundation of Anhui Province [1408085MKL52]
Fluorescent test papers are promising for the wide applications in the assays of diagnosis, environments and foods, but unlike classical dye-absorption-based pH test paper, they are usually limited in the qualitative yes/no type of detection by fluorescent brightness, and the colorimetry-based quantification remains a challenging task. Here, we report a single dual-emissive nanofluorophore probe to achieve the consecutive color variations from blue to red for the quantification of blood glucose on its as-prepared test papers. Red quantum dots were embedded into silica nanoparticles as a stable internal standard emission, and blue carbon dots (CDs) were further covalently linked onto the surface of silica, in which the ratiometric fluorescence intensity of blue to red is controlled at 5:1. While the oxidation of glucose induced the formation of Fe3+ ions, the blue emission of CDs was thus quenched by the electron transfer from CDs to Feat, displaying a serial of consecutive color variations from blue to red with the dosage of glucose. The high-quality test papers printed by the probe ink exhibited a dosage-sensitive allochromatic capability with the clear differentiations of 5, 7, 9, 11 mM glucose in human serum (normal: 3-8 mM). The blood glucose determined by the test paper was almost in accordance with that measured by a standard glucometer. The method reported here opens a window to the wide applications of fluorescent test paper in biological assays. (C) 2016 Elsevier B.V. All rights reserved.
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