Associations between metal exposure and global DNA methylation in potentially affected people in E-Waste recycling sites in Taizhou City, China

Electronic waste (e-waste) has been an emerging environmental health issue, and it has already provoked all aspects of attention. Taizhou is one of the three largest e-waste recycling locations in China. At present, to prevent the environmental problems stem from e-waste dismantling, the local government has shut down all the industries in 2015. In this study, we collected blood samples of residents living near e-waste dismantling factories, and in matched reference areas in Taizhou, in December 2017, after the factories have been shut down for two years. Twenty-five metals were quantified in all blood samples. Among them, the concentrations of As, Ni, Ag, La, and Ce were statistically significant higher in individuals in e-waste recycling locations than those in reference location. Global DNA methylation was measured in blood as a marker of human health. Pearson correlation and multiple linear regression analysis between the changed metals and global DNA methylation in blood were performed. The result showed that only blood Ce was negatively correlated with global DNA methylation level significantly in pre-workers exposed e-waste workers (r = -0.51, p = 0.01). Our findings indicated that high concentrations of exposure to Ce in e-waste dismantling site could have sustained effects on the DNA methylation in blood although the e-waste industry had been closed for 2 years. (C) 2019 Elsevier B.V. All rights reserved.