期刊
BIOSENSORS & BIOELECTRONICS
卷 77, 期 -, 页码 631-637出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.10.030
关键词
Impedance; MMP-9; Capacitance; Interdigitated electrode; Proteolytical cleavage; ECIS
类别
资金
- BioNano Health-Guard Research Center
- Ministry of Science, ICT & Future Planning (MSIP) of Korea as Global Frontier Project [H-GUARD_2015M3A6B2063548]
- Nano Material Technology Development Program through the National Research Foundation of Korea (NRF)
- Korea government (MSIP) [NRF-2014M3A7B4051907]
MMP-9 (92 kDa gelatinease), which is member of matrix metalloproteinases (MMPs) family, plays a crucial role in the breakdown of extracellular matrix (ECM) by degrading the major components of ECM that lead to tumor cell invasion and metastasis through the basement membrane. Our study presents the on-chip dual-sensing device for rapid detection of cell-secreted MMP-9 and corresponding cell morphology changes in real-time domain. The device consists of 2 sensing platforms (both are interdigitated array microelectrodes - IDAMs) within 1 common fluidic chamber: one detects the cell morphology responses via Electric Cell-substrate Impedance Sensing (ECIS) technique, meanwhile the other records the cleavage effect between cell-secreted MMP-9 and the surface immobilized peptide via the capacitance-based sensing method. Thanks to the selectivity of designed peptide, this approach allows the rapid and specific detection of MMP-9. In comparison with gold standard ELISA assay, the detection time was significantly reduced from over 4 h to within 30 min with the wide detection range from 10 pM to 10 nM. Finally, this study provides the novel model for MMP-9 protease direct detection from living cell and new insights in multi-purpose detection of cancer associated enzyme and cell migration behavior. (C) 2015 Elsevier B.V. All rights reserved.
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