期刊
BIOSENSORS & BIOELECTRONICS
卷 77, 期 -, 页码 752-758出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.10.050
关键词
Electrochemical aptasensor; Thionine; Platinum; Kanamycin
类别
资金
- Shandong Provincial Natural Science Foundation, China [ZR2012BL11]
- Shandong Provincial Science and Technology Development Plan Project, China [2013GGX10705]
An ultrasensitive electrochemical aptasensor for the quantitative detection of kanamycin antibiotic was fabricated based on a novel signal amplification strategy. This aptasensor was developed using thionine functionalized graphene (GR-TH) and hierarchical nanoporous (HNP) PtCu alloy as biosensing substrates for the first time. HNP-PtCu alloy with controllable bimodal ligament/pore distributions was successfully prepared by two-step dealloying of a well-designed PtCuAl precursor alloy combined with an annealing operation. GR-TH composite was synthesized by one-step reduction of graphene oxide (GO) in TH solution. Greatly amplified sensitivity was achieved by using GR-TH/FINP-PtCu composite owing to its large specific surface and good electron-transfer ability. Under the optimized conditions, the proposed aptasensor exhibited a high sensitivity and a wider linearity to kanamycin in the range 5 x 10(-7)-5 x 10(-2) mu g mL(-1) with a low detection limit of 0.42 pg mL(-1). This aptasensor also displayed a satisfying electrochemical performance with good stability, selectivity and reproducibility. The as-prepared aptasensor was successfully used for the determination of kanamycin in animal derived food. (C) 2015 Elsevier B.V. All rights reserved.
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