期刊
BIOSENSORS & BIOELECTRONICS
卷 77, 期 -, 页码 740-745出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.10.047
关键词
Biosensor; Hg(II); DNA charge transfer; Electrochemiluminescence; Quantum dots; Graphene
类别
资金
- Foundation of National Natural Science Foundation of China [21005005, 21375005, 21475006]
- Beijing Nova Program [2010B009]
- Program for New Century Excellent Talents in University [NCET-12-0603]
In this article, we developed an electrochemiluminescence (ECL) sensor with a high-intensity charge transfer interface for Hg2+ detection based on Hg(II)-induced DNA hybridization. The sensor was fabricated by the following simple method. First, graphene oxide (GO) was electrochemically reduced onto a glassy carbon electrode through cyclic voltammetry. Then, amino-labeled double-stranded (ds)DNA was assembled on the electrode surface using 1-pyrenebutyric acid N-hydroxysuccinimide as a linker between GO and DNA. The other terminal of dsDNA, which was labeled with biotin, was linked to CdSe quantum dots via biotin-avidin interactions. Reduced graphene oxide has excellent electrical conductivity. dsDNA with T-Hg(II)-T base pairs exhibited more facile charge transfer. They both accelerate the electron transfer performance and sensitivity of the sensor. The increased ECL signals were logarithmically linear with the concentration of Hg(II) when Hg2+ was present in the detection solution. The linear range of the sensor was 10(-11) to 10(-8) mol/L (R=0.9819) with a detection limit of 10(-11) mol/L. This biosensor exhibited satisfactory results when it was used to detect Hg(II) in real water samples. The biosensor with high-intense charge transfer performance is a prospect avenue to pursue more and more sensitive detection method. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据