期刊
BIOSENSORS & BIOELECTRONICS
卷 77, 期 -, 页码 624-630出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.10.019
关键词
Molecular imprinting; Ratiometric fluorescence; Fluorescence resonance energy transfer; Phycocyanin
类别
资金
- National Natural Science Foundation of China [21175084, 21275091, 21275158, 21477160]
- Research Fund for the Doctoral Program of Higher Education of China [20113704110003]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
A facile strategy was developed to prepare molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin (PC) based on fluorescence resonance energy transfer (FRET), via a sal-gel polymerization process using nitrobenzoxadiazole (NBD) as fluorescent signal source. The ratio of two fluorescence peak emission intensities of NBD and PC was utilized to determine the concentration of PC, which could effectively reduce the background interference and fluctuation of diverse conditions. As a result, this sensor obtained high sensitivity with a low detection limit of 0.14 nM within 6 min, and excellent recognition specificity for PC over its analogues with a high imprinting factor of 9.1. Furthermore, the sensor attained high recoveries in the range of 93.8-110.2% at three spiking levels of PC, with precisions below 4.7% in seawater and lake water samples. The developed sensor strategy demonstrated simplicity, reliability, rapidity, high selectivity and high sensitivity, proving to be a feasible way to develop high efficient fluorescence sensors and thus potentially applicable for ultratrace analysis of complicated matrices. (C) 2015 Elsevier B.V. All rights reserved.
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