期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 705, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2019.135674
关键词
Antibiotic resistome; Gut microbiota; Antibiotic therapy; Chinese patient
资金
- Key projects of the National Natural Science Foundation of China [41831287]
- China National Science Fund for Distinguished Young Scholars [41525013]
- National Natural Science Foundation of China [31870351, 31670509, 41807483, 21607016, 41703088, 41977367]
- Key Projects of Research and Development of Hebei Province [19273707D]
- 111 Program, Ministry of Education, China [T2017002]
- Fundamental Research Funds for the Central Universities
Human gut microbiota is an important reservoir of antibiotic resistance genes (ARGs). Although dysbacteriosis after the antibiotic course has been previously observed in the patient guts, a comprehensive comparison of gut resistomes, microbiota and antibiotic residues in healthy individuals and patients undergoing antibiotic administration is little. Using high-throughput qPCR, 16S rRNA gene amplicon sequencing and UPLC-MS/MS, we systematically examined the antibiotic resistome, gut microbiota, and antibiotic residues in fecal samples from both Chinese healthy individuals and patients receiving antibiotic therapy. Compared with healthy individuals, patients guts harbored lower diverse gut resistome and microbiota, but higher concentrations of antibiotics and ARGs. Antibiotic concentration in human guts was positively correlated with ARG total abundance, but was negatively related to the diversity of both ARGs and bacterial communities, which demonstrated that antibiotic administration could shape the antibiotic resistomes and bacterial communities in the patient guts. Gene OA was evaluated as a potential bio-marker to distinguish the patients receiving antibiotic therapy from the healthy individuals in China since its wide detection and significant enrichment in the guts of the patients. The detection of some veterinary antibiotics in human guts illustrated the potential transmission of antibiotic from the external environment to human via the food chain. The obtained results could help to better understand the influence of antibiotic therapy in shaping antibiotic reistomes and bacterial communities in Chinese individuals. (C) 2019 Published by Elsevier B.V.
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