期刊
BIOSENSORS & BIOELECTRONICS
卷 77, 期 -, 页码 51-55出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.09.012
关键词
GOx-HRP nanoflowers; Enzymatic microreactor; mu PADs; Colorimetric detection; Glucose monitoring
类别
资金
- Spanish MINECO [CTQ2013-44545-R]
- Junta de Andalucia, Spain [P10-FQM-5974]
- Programs Fortalecimiento de Grupos and Reincorporacion de Doctores
- ERDF
This work reports on the development of a 3D microfluidic paper-based device (3D mu PAD) for glucose detection using organic-inorganic hybrid nanoflower technology to immobilize the bi-enzymatic system (glucose oxidase and horseradish peroxidase). The system is based on nanoflowerssupported on cellulose paper (the microreactor zone) coupled to 3,3',5,5'-tetramethylbenzidine (TMB) as the colorimetric probe in the detection zone. We used a digital camera for the quantitative analysis of glucose with the S coordinate of the HSV color space as the analytical parameter. Under optimal operational conditions, linearity was observed for glucose concentrations up to 300 mu M, with a detection limit of 15.6 mu M. The biosensor is reusable and remains stable for 75 days in conventional storage conditions. (C) 2015 Elsevier B.V. All rights reserved.
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